PARIS (AFP) - AC Milan and defending champions Barcelona both reached the last 16 of the Champions League on Tuesday, but Premier League giants Arsenal and Chelsea were made to wait to secure qualification. Arsenal, 5-3 victors at Chelsea on Sunday, needed victory at home to Marseille to book their place in the knockout phase but they were held to a goalless draw. The hosts enjoyed the best of the first half, with OM goalkeeper Steve Mandanda saving well from Theo Walcott and Gervinho, while Aaron Ramsey volleyed over from close range. Arsenal could not sustain their momentum after the interval, however, and even the introduction of in-form captain Robin van Persie as a substitute was unable to illuminate the path to goal. Its difficult to be at your maximum three days after a big match, explained Arsenal manager Arsene Wenger. Marseille played a good, very solid match and I think their point was not undeserved. The result preserved Arsenals one-point lead over second-placed Marseille in Group F, while Borussia Dortmund resurrected their qualifying hopes with a 1-0 win at home to Olympiakos that took them to within three points of OM. Chelsea were held to a 1-1 draw at Belgian champions Genk, although Valencias 3-1 defeat of Bayer Leverkusen meant that the Blues would not have been able to qualify in any case. Ramires put Chelsea ahead in the 26th minute, arrowing home from a narrow angle from Fernando Torres pass, before David Luiz saw a penalty saved by Laszlo Koteles after Thomas Buffel handled Florent Maloudas cross. Chelsea had thrashed Genk 5-0 in their previous encounter at Stamford Bridge but they were made to rue Luizs miss just after the hour when Jelle Vossen swept home the equaliser from Fabien Camus cut-back. At the moment, its not happening for us, said Chelsea coach Andre Villas-Boas. Its a fixture I wanted to win. With a draw, not everything is negative but we could have done better. Valencia scored the second-fastest goal in competition history against Leverkusen, with Jonas netting after 10.6 seconds at the Mestalla 0.3sec slower than Roy Makaays effort for Bayern Munich against Real Madrid in 2007. Stefan Kiessling drew the German side level on the half hour but Roberto Soldado flicked home Jeremy Mathieus cross to restore Valencias lead in the second half before Adil Rami made it 3-1 with 15 minutes to play. Chelsea now lead Leverkusen by two points at the top of Group E, while Valencias qualification hopes have been rekindled as they are now just a point beneath the top two ahead of a home game with Genk on November 23. Lionel Messi sent fresh landmarks tumbling as he netted another superb hat-trick in a 4-0 success for Barcelona at 10-man Czech champions Viktoria Plzen. The Argentine broke the deadlock from the penalty spot in the 24th minute, after Marian Cisovsky had been sent off for bringing him down. The spot-kick took Messis tally for the club to 200 goals and his second goal, in first-half injury time, was his 50th goal for club and country in 2011. Cesc Fabregas headed home in the 72nd minute, with Messi completing his second hat-trick in the space of four days after Saturdays triple against Real Mallorca in injury time. The win sent Pep Guardiolas side two points above Milan in Group H, after the Italian champions earlier 1-1 draw.